Abstract：Pseudomonas monteilii TA-5 cultivated with LB medium was employed as the biocatalysts（Cat) for the synthesis of (S)-(+)-4-phenyl-2-butanol with high enantioselectivity by asymmetric reduction. In this paper, the optimal reaction conditions for bioreduction of 4-phenyl-2-butanone（1） were optimized. Under the optimized reaction conditions［1 10 mmol·L-1, c(Cat) 30 g·cdw·L-1, 10% glycerol as the cosolvent, pH 8.0, 300 r·min-1, at 30 ℃ for 4 h］, the conversion was 82% and the ee was 91%.
杨敏, 李珂, 周晓建, 崔宝东, 陈永正. 微生物酶催化的不对称还原反应合成(S)-(+)-4-苯基-2-丁醇[J]. 合成化学, 2016, 24(3): 227-230.
YANG Min, LI Ke, ZHOU Xiao-jian, CUI Bao-dong, CHEN Yong-zheng. Asymmetric Synthesis of (S)-(+)-4-phenyl-2-butanol by Carbonyl Reduction with Microbial Enzymes. Chinese Journal of Synthetic Chemistry, 2016, 24(3): 227-230.
2016, Vol. 24 
